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alchetron.com
Lysis buffer - Alchetron, The Free Social Encyclopedia
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the compounds of the cells (e.g. western blot). Most lysis buffers contain salts (e.g. TrisHCl or EDTA) to regulate the acidity and osmolarity of the lysate. Sometim
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SOLVED: 1. What does the lysis buffer do? (after resuspending the cells, add lysis buffer)2. What is left out of the water control for PCR? (Restriction mapping and Ligation)3. Would you expect the plasmid that was restricted by two different restriction enzymes to be recircularized by ligase? Why or why not?
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